PKD2 Flox; CAGG-CreERT2

Description: This strain carries the PKD2 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion of PKD2.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request.

PKD2 Flox; GCaMP6F

Description: This strain carries the PKD2 Flox allele crossed with the GCamp6F cytosolic targeted Ca2+ biosensor.
Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

PKD2 Flox; CAGG-CreERT2; Rosa26

Description: This strain carries the PKD2 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion, and the Rosa26 LacZ Cre reporter to detect Cre activity.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD2 Flox; Ksp-Cre

Description: This strain has the PKD1 Flox allelle crossed with the Ksp (Cadherin 16) promoter driven Cre recombinase. Cre-mediated recombination is detected in the epithelium of the kidney, the genitourinnary tract, and adult renal tubules especially the collecting ducts, loops of Henle and distal tubules.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD2 Flox; Pax8-rtTA; tetO-7-Cre

Description: This strain caries the PKD2 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre. This allows tetracycline/doxycycline induced deletion of PKD2 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney

Contact: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

PKD2 Flox; Pax8-rtTA; tetO-7-Cre; mTmG

Description: This strain caries the PKD2 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre and the mTmG Cre reporter. Treatment with tetracycline/doxycycline induces deletion of PKD2 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. Cre-recombinase activity is reflected by a switch from expression of mTomato prior to mGFP.

Contact
: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

PKD2 Flox; Ift88 Flox; CAGG-CreERT2

Description: This strain carries both the PKD2 and Ift88 Floxed alleles crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion of both genes.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD2 Flox; PKHD1-Cre

Description: This strain caries the PKD2 Floxed allele crossed with the PKDHd1 promoter that drives Cre expression predominantly in the collecting duct.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD2 Flox; Ift88 Flox; mTmG

Description: This strain carries both the PKD2 and Ift88 Floxed alleles crossed with mTmG Cre reporter to detect Cre activity. Prior to Cre recombinase activation, the cells express mTomato. After Cre excision of mTomato, the cells express mGFP.
Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

PKD1 Flox; CAGG-CreERT2; Rosa26

Description: This strain carries the PKD1 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion, and the Rosa26 LacZ Cre reporter to detect Cre activity.

Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

PKD1flox; KspCre

Description: This strain has the Pkd1 Flox allele crossed with the Ksp (Cadherin 16) promoter driven Cre recombinase. Cre-mediated recombination is detected in the epithelium of the kidney, the genitourinary tract, and adult renal tubules especially the collecting ducts, loops of Henle and distal tubules.

Contact: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

PKD1flox; mTmG

Description: This strain carries the PKD1 Floxed allele crossed with the mTmG Cre reporter. Prior to Cre-mediated recombination, there is expression of mTomato. After Cre mediated excision there is expression of mGFP.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD1 Flox; Pax8-rtTA; tetO-7-Cre

Description: This strain caries the PKD1 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre. This allows tetracycline/doxycycline induced deletion of PKD1 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

PKD1 Flox; Pax8-rtTA; tetO-7-Cre; mTmG

Description: This strain caries the PKD1 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre and the mTmG Cre reporter. Treatment with tetracycline/doxycycline induces deletion of PKD1 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. Cre-recombinase activity is reflected by a switch from expression of mTomato prior to mGFP.

Contact
: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

PKD1 Flox; PKHD1-Cre

Description: This strain carries the Pkd1 Floxed allele crossed with the Pkdhd1 promoter that drives Cre expression predominantly in the collecting duct.

Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

PKD2 Flox; mTmG

Description:This strain carries the PKD2 Floxed allele crossed with the mTmG Cre reporter. Prior to Cre-mediated recombination, there is expression of mTomato. After Cre mediated excision there is expression of mGFP.
Contact: pgarcia@som.umaryland.edu, pydnam@uab.edu
Note : Additional combination of alleles can be performed upon request

PKD2 Flox; 5HT6mCherry-IA-G-GECO

Description: This strain carries the PKD2 Floxed allele crossed with the 5HT6mCherry-IA-G-Geco ciliary targeted ratiometric Ca2+ biosensor to measure calcium changes in the cilium.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

NPHP14 Null

Allele Description: Null allele of NPHP14 (Znf423)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are lethal. Maintained as a heterozygous stock.
Background: AB
Contact: info@pkd-rrc.org

PKD1 flox

Description: This conditional PKD1 floxed mouse model harbors LoxP sites flanking exons 2 through 4. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of PKD1 in the Cre targeted tissue.
Contact: info@pkd-rrc.org

Rosa26 LacZ Reporter

Description: This mouse reporter line harbors a lox-P flanked neomycin cassette up-stream of a β-galactosidase (Lac-Z) sequence. These mice express Lac-Z following Cre-mediated recombination.
Contact: info@pkd-rrc.org

PKD1 delta L

Description: This knock-in mutant mouse model mimics a patient-derived mutation with a deletion of Leucine at position 4122. Homozygous mice are embryonically lethal.

Contact: info@pkd-rrc.org

Rosa26-M2rtTA

Description: This transgenic mouse model allows widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein inserted downstream of ROSA promoter. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models. Homozygous and hemizygous mice are phenotypically normal.

Contact: info@pkd-rrc.org

PKD1 3xHA

Description: This knock-in mouse model produces a fully functional polycystin 1 protein with a C-terminal triple HA tag. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

(tetO)7-Cre

Description: This transgenic mouse model expresses Cre recombinase under the control of a tetracycline-responsive promoter element (TRE or tetO) and is an effective tool for inducible, tissue-specific studies. When bred with another transgenic mouse expressing the reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) the Cre mediated recombination is induced by doxycycline.
Contact: info@pkd-rrc.org

Rosa26-FlpoER

Description: This transgenic mouse model has a tamoxifen-inducible Cre-mediated recombination system driven by the chicken beta actin promoter and a tamoxifen-inducible FlpoER fusion protein inserted into the Gt(ROSA)26Sor locus, resulting in FLP recombination in all embryonic and adult tissues containing frt-flanked sequences. Homozygous and hemizygous mice are phenotypically normal.

Contact: info@pkd-rrc.org

Ift88 Orpk FVB

Description: This mouse model harbors a transgene that deletes 2.7kb of intron 23 in the Ift88 gene resulting in a null allele. Heterozygous mice are phenotypically normal. Homozygous mice in the FVB background develop multiple embryonic defects including preaxial polydactyly, hydrocephalus and cystic kidneys and can survive to 7-21 days of age.
Contact: info@pkd-rrc.org

Note: Additional combinations of alleles are available upon request

JBTS13 Null

Strain: Tctn1
ZFIN ID:ZDB-GENE-081104-157
Allele designation: Insertion of 5 bp; frameshift in exon 1
Allele Description: Null allele of Tctn1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

R26tdTomato Reporter

Description: This mouse reporter line harbors a lox-P flanked STOP cassette preventing transcription of a CAG promoter driven red fluorescent protein (tdTomato) in the Rosa26 locus. These mice express robust tdTomato fluorescence following Cre-mediated recombination.
Contact: info@pkd-rrc.org

Nphp4 Truncation

Description: This mouse model carry an ENU-induced mutation in the Nphp4 gene. Heterozygous mice are phenotypically normal. Homozygous mice develop a severe photoreceptor degeneration in the eye, oligozoospermia and asthenozoospermia.
Contact: info@pkd-rrc.org

Note: Additional combinations of alleles are available upon request

Rosa26-CreER

Description: These mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mice are viable and fertile and phenotypically normal.
Contact: info@pkd-rrc.org

Nphp4 Allele 2

Allele Description: Null allele #2 of Nphp4
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

PKHD1 3xHA

Description: This knock-in mouse model has a triple HA tag at the C-terminal tail of PKHD1 and produces a fully functional fibrocystin protein. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

PKHD1 Delta 3-4

Description: This mouse model has a deletion of PKHD1 exons 3 through 4. Heterozygous mice are phenotypically normal. Homozygous mice develop congenital hepatic fibrosis.

Contact: info@pkd-rrc.org

PKHD1 Delta 67

Description: This mouse model lacks PKHD1 exon 67. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

PKD2 Heterozygous

Description: This knock-out mouse model has a deletion of PKD2 exons 11 through 13. Heterozygous mice are phenotypically normal. Homozygous mice are embryonically lethal at mid-gestation.
Contact: info@pkd-rrc.org

PKD2 flox

Description: This conditional PKD2 floxed mouse model harbors LoxP sites flanking exons 11 through 13. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of PKD2 in the Cre targeted tissue.
Contact: info@pkd-rrc.org

PKD1 V

Description: This knock-in mouse model has an amino acid substitution at position T3041V of the G-protein coupled receptor proteolytic site (GPS) of polycystin 1, disrupting normal cleavage. Heterozygous mice are phenotypically normal. Homozygotes are phenotypically normal at birth but develop cysts and die within 2-3 weeks.
Contact: info@pkd-rrc.org

PKD1 N Delta L

Description: This knock-in mutant mouse model has a Neomycin targeting cassette disrupting splicing of exon 45 and a deletion of Leu 4122 in PKD1. The splice defect results in premature truncation of the mutant protein. Homozygous mice are embryonically lethal. Cre-mediated excision of the targeting cassette produces the PKD1 Delta L allele.

Contact: info@pkd-rrc.org

PKD1 Heterozygous

Description: This knock-out mouse model has a deletion of PKD1 exons 2 through 4. Heterozygous mice are phenotypically normal. Homozygous mice are embryonically lethal at mid-gestation.
Contact: info@pkd-rrc.org

Mks1 Null

Strain: Mks1
ZFIN ID:ZDB-GENE-030131-3813
Allele designation: Insertion of 2 bp, frameshift in exon 2
Allele: Null allele of Mks1
Background: AB
Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org

Mks2 Null

Strain: Mks2 (tmem216)
ZFIN ID:ZDB-GENE-100818-1
Allele designation:Δ8 bp; frameshift in exon 4
Allele Description:Null allele of Mks2 (tmem216)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Background: AB
Contact: info@pkd-rrc.org

Mks3 Null

Allele Description: Null allele of Mks3 (tmem67)

Phenotype Description: Heterozygous mutant zebrafish are viable. Majority of homozygous mutants are embryonic lethal, however some live and have spine curvature defects. These viable mutants are infertile. Maintained as a heterozygous stock

Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Mks6 Null

Allele Description:Null allele of Mks6 (cc2d2a)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Nphp4 Null

Allele Description: Null allele #1 of Nphp4
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

Mks5 Null

Allele Description: Null allele of Mks5 (rpgrip1l; nphp8)
Phenotype Description: Heterozygous mutant zebrafish are viable. Some of the homozygous mutant zebrafish survive and some have slight spine curvature defects. These viable mutants have reduced fertility. Maintained as a heterozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Nphp1 Allele 2

Allele Description: Null allele #2 of Nphp1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

Nphp1 Null

Allele Description: Null allele #1 of Nphp1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org