Pkd1 Flox; HoxB7-Cre: Deletion of Pkd1 in tubular segments derived from the ureteric bud

Description: These mice strain carries the PKD1 Floxed allele crossed with a transgenic mouse model expresses Cre recombinase under the control of the mouse HoxB7 promoter. The Cre recombinase expression is detected in collecting ducts of ureteric bud descendants.

Contact: info@pkd-rrc.org

Pkd2 Flox; Rosa26-CreER: Global deletion of Pkd2 after tamoxifen induction

Description: These mice strain carries the PKD2 Floxed allele crossed with a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mice are viable and fertile and phenotypically normal.

Contact: info@pkd-rrc.org

Pkd1 Flox; Rosa26-CreER: Allows global deletion of Pkd1 via tamoxifen injection

Description: These mice strain carries the PKD1 Floxed allele crossed with a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mice are viable and fertile and phenotypically normal. Depending on the timing of tamoxifen induction this will lead to a either a rapidly or slowly progressing model of PKD.

Contact: info@pkd-rrc.org

Ift88flox;GCaMP6F

Contact: pydnam@uab.edu
Note: Additional combinations of alleles are available upon request

Ift88flox;mTmG

Contact: pydnam@uab.edu
Note: Additional combinations of alleles are available upon request

Pkd2 Flox; Pax8-rtTA; tetO-7-Cre; mTmG: Nephron specific deletion of Pkd2 after Doxycycline induction with a fluorescent reporter

Description: This strain caries the PKD2 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre and the mTmG Cre reporter. Treatment with tetracycline/doxycycline induces deletion of PKD2 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. Cre-recombinase activity is reflected by a switch from expression of mTomato prior to mGFP.

Contact
: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

Pkd2 Flox; Pkhd1-Cre: Collecting duct specific deletion of Pkd2

Description: This strain caries the PKD2 Floxed allele crossed with the PKDHd1 promoter that drives Cre expression predominantly in the collecting duct.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

Pkd2 Flox; Ift88 Flox; CAGG-CreERT2

Description: This strain carries both the PKD2 and Ift88 Floxed alleles crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion of both genes.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

Ift88flox;CAGGCre-ER

Contact: pydnam@uab.edu
Note: Additional combinations of alleles are available upon request

Pkd2 Flox; Ift88 Flox; mTmG

Description: This strain carries both the PKD2 and Ift88 Floxed alleles crossed with mTmG Cre reporter to detect Cre activity. Prior to Cre recombinase activation, the cells express mTomato. After Cre excision of mTomato, the cells express mGFP.
Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

Pkd1 Flox; CAGG-CreERT2; Rosa26: Ubiquitous deletion of Pkd1 after Tamoxifen induction

Description: This strain carries the PKD1 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion, and the Rosa26 LacZ Cre reporter to detect Cre activity.

Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

Pkd1 Flox; KspCre: Distal and collecting duct specific deletion of Pkd1

Description: This strain has the Pkd1 Flox allele crossed with the Ksp (Cadherin 16) promoter driven Cre recombinase. Cre-mediated recombination is detected in the epithelium of the kidney, the genitourinary tract, and adult renal tubules especially the collecting ducts, loops of Henle and distal tubules.

Contact: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request. Possible delay due to third party MTA requirement.

Pkd1flox; mTmG: Pkd1 conditional allele with a fluorescent reporter

Description: This strain carries the PKD1 Floxed allele crossed with the mTmG Cre reporter. Prior to Cre-mediated recombination, there is expression of mTomato. After Cre mediated excision there is expression of mGFP.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

Pkd1 Flox; Pax8-rtTA; tetO-7-Cre: Nephron specific deletion of Pkd1 after Doxycycline induction

Description: This strain caries the PKD1 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre. This allows tetracycline/doxycycline induced deletion of PKD1 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

Pkd1 Flox; Pax8-rtTA; tetO-7-Cre; mTmG: Nephron specific deletion of Pkd1 after Doxycycline induction with a fluorescent reporter

Description: This strain caries the PKD1 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre and the mTmG Cre reporter. Treatment with tetracycline/doxycycline induces deletion of PKD1 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. Cre-recombinase activity is reflected by a switch from expression of mTomato prior to mGFP.

Contact
: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

Pkd1 Flox; PKHD1-Cre: Collecting duct specific deletion of Pkd1

Description: This strain carries the Pkd1 Floxed allele crossed with the Pkhd1 promoter that drives Cre expression predominantly in the collecting duct.

Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

Pkd2 Flox; mTmG: Pkd2 conditional allele with a fluorescent reporter

Description:This strain carries the PKD2 Floxed allele crossed with the mTmG Cre reporter. Prior to Cre-mediated recombination, there is expression of mTomato. After Cre mediated excision there is expression of mGFP.
Contact: pgarcia@som.umaryland.edu, pydnam@uab.edu
Note : Additional combination of alleles can be performed upon request

PKD2 Flox; CAGG-CreERT2

Description: This strain carries the PKD2 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion of PKD2.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request.

Pkd2 Flox; GCaMP6F

Description: This strain carries the PKD2 Flox allele crossed with the GCamp6F cytosolic targeted Ca2+ biosensor.
Contact: info@pkd-rrc.org

Note: Additional combination of alleles can be performed upon request

Pkd2 Flox; CAGG-CreERT2; Rosa26: Ubiquitous deletion of Pkd2 after Tamoxifen induction

Description: This strain carries the PKD2 Floxed allele crossed with CAGG-CreERT2 for systemic tamoxifen inducible deletion, and the Rosa26 LacZ Cre reporter to detect Cre activity.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request

Pkd2 Flox; Ksp-Cre: Distal and collecting duct specific deletion of Pkd2

Description: This strain has the PKD1 Flox allelle crossed with the Ksp (Cadherin 16) promoter driven Cre recombinase. Cre-mediated recombination is detected in the epithelium of the kidney, the genitourinnary tract, and adult renal tubules especially the collecting ducts, loops of Henle and distal tubules.
Contact: info@pkd-rrc.org

Note : Additional combination of alleles can be performed upon request. Possible delay due to third party MTA requirement.

Pkd2 Flox; Pax8-rtTA; tetO-7-Cre: Nephron specific deletion of Pkd2 after Doxycycline induction

Description: This strain caries the PKD2 Floxed allele crossed with Pax8-rtTA; tetO-7-Cre. This allows tetracycline/doxycycline induced deletion of PKD2 in the proximal and distal tubules and the collecting duct system of both embryonic and adult kidney

Contact: info@pkd-rrc.org

Note
: Additional combination of alleles can be performed upon request

NPHP14 Null

Allele Description: Null allele of NPHP14 (Znf423)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are lethal. Maintained as a heterozygous stock.
Background: AB
Contact: info@pkd-rrc.org

JBTS13 Null

Strain: Tctn1
ZFIN ID:ZDB-GENE-081104-157
Allele designation: Insertion of 5 bp; frameshift in exon 1
Allele Description: Null allele of Tctn1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

Rosa26 LacZ Reporter: Expresses LacZ after Cre recombination

Description: This mouse reporter line harbors a lox-P flanked neomycin cassette up-stream of a β-galactosidase (Lac-Z) sequence. These mice express Lac-Z following Cre-mediated recombination.
Contact: info@pkd-rrc.org

Pkd1 deltaL: Pkd1 patient mutation L4122delta

Description: This knock-in mutant mouse model mimics a patient-derived mutation with a deletion of Leucine at position 4122. Homozygous mice are embryonically lethal.

Contact: info@pkd-rrc.org

Rosa26-M2rtTA: Ubiquitous expression of tet-responsive rtTA

Description: This transgenic mouse model allows widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein inserted downstream of ROSA promoter. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models. Homozygous and hemizygous mice are phenotypically normal.

Contact: info@pkd-rrc.org

Pkd1 3xHA: Endogenously tagged Pkd1 allele

Description: This knock-in mouse model produces a fully functional polycystin 1 protein with a C-terminal triple HA tag. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

Rosa26-FlpoER: Ubiquitous expression of tamoxifen inducible Flp recombinase

Description: This transgenic mouse model has a tamoxifen-inducible Cre-mediated recombination system driven by the chicken beta actin promoter and a tamoxifen-inducible FlpoER fusion protein inserted into the Gt(ROSA)26Sor locus, resulting in FLP recombination in all embryonic and adult tissues containing frt-flanked sequences. Homozygous and hemizygous mice are phenotypically normal.

Contact: info@pkd-rrc.org

Ift88 Orpk FVB

Description: This mouse model harbors a transgene that deletes 2.7kb of intron 23 in the Ift88 gene resulting in a null allele. Heterozygous mice are phenotypically normal. Homozygous mice in the FVB background develop multiple embryonic defects including preaxial polydactyly, hydrocephalus and cystic kidneys and can survive to 7-21 days of age.
Contact: info@pkd-rrc.org

Note: Additional combinations of alleles are available upon request

R26tdTomato Reporter: Expresses tdTomato after Cre recombination

Description: This mouse reporter line harbors a lox-P flanked STOP cassette preventing transcription of a CAG promoter driven red fluorescent protein (tdTomato) in the Rosa26 locus. These mice express robust tdTomato fluorescence following Cre-mediated recombination.
Contact: info@pkd-rrc.org

Nphp4 Truncation

Description: This mouse model carry an ENU-induced mutation in the Nphp4 gene. Heterozygous mice are phenotypically normal. Homozygous mice develop a severe photoreceptor degeneration in the eye, oligozoospermia and asthenozoospermia.
Contact: info@pkd-rrc.org

Note: Additional combinations of alleles are available upon request

Rosa26-CreER: Ubiquitous expression of tamoxifen inducible Cre

Description: These mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mice are viable and fertile and phenotypically normal.
Contact: info@pkd-rrc.org

Nphp4 Allele 2

Allele Description: Null allele #2 of Nphp4
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Pkhd1 3xHA: Endogenously HA-tagged Pkhd1 model ARPKD

Description: This knock-in mouse model has a triple HA tag at the C-terminal tail of PKHD1 and produces a fully functional fibrocystin protein. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

Pkhd1 Delta 3-4: Pkhd1 mutant model ARPKD

Description: This mouse model has a deletion of PKHD1 exons 3 through 4. Heterozygous mice are phenotypically normal. Homozygous mice develop congenital hepatic fibrosis.

Contact: info@pkd-rrc.org

Pkhd1 Delta 67: Pkhd1 mutant model ARPKD

Description: This mouse model lacks PKHD1 exon 67. Homozygous mice are phenotypically normal.
Contact: info@pkd-rrc.org

Pkd2 Heterozygous: Pkd2 knock-out allele

Description: This knock-out mouse model has a deletion of PKD2 exons 11 through 13. Heterozygous mice are phenotypically normal. Homozygous mice are embryonically lethal at mid-gestation.
Contact: info@pkd-rrc.org

Pkd2 Flox: Pkd2 conditional allele

Description: This conditional PKD2 floxed mouse model harbors LoxP sites flanking exons 11 through 13. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of PKD2 in the Cre targeted tissue.
Contact: info@pkd-rrc.org

Pkd1 V: Uncleavable Pkd1 hypomorhic allele

Description: This knock-in mouse model has an amino acid substitution at position T3041V of the G-protein coupled receptor proteolytic site (GPS) of polycystin 1, disrupting normal cleavage. Heterozygous mice are phenotypically normal. Homozygotes are phenotypically normal at birth but develop cysts and die within 2-3 weeks.
Contact: info@pkd-rrc.org

PKD1 N Delta L: Pkd1 C-terminal truncation allele

Description: This knock-in mutant mouse model has a Neomycin targeting cassette disrupting splicing of exon 45 and a deletion of Leu 4122 in PKD1. The splice defect results in premature truncation of the mutant protein. Homozygous mice are embryonically lethal. Cre-mediated excision of the targeting cassette produces the PKD1 Delta L allele.

Contact: info@pkd-rrc.org

Pkd1 Heterozygous: Pkd1 knock-out allele

Description: This knock-out mouse model has a deletion of PKD1 exons 2 through 4. Heterozygous mice are phenotypically normal. Homozygous mice are embryonically lethal at mid-gestation.
Contact: info@pkd-rrc.org

PKD1 flox: Pkd1 conditional allele

Description: This conditional PKD1 floxed mouse model harbors LoxP sites flanking exons 2 through 4. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of PKD1 in the Cre targeted tissue.
Contact: info@pkd-rrc.org

Nphp4 Null

Allele Description: Null allele #1 of Nphp4
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

Mks6 Null

Allele Description:Null allele of Mks6 (cc2d2a)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Nphp1 Allele 2

Allele Description: Null allele #2 of Nphp1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Background: AB
Contact: info@pkd-rrc.org

HoxB7-Cre: Expresses Cre in collecting ducts

Description: This transgenic mouse model expresses Cre recombinase under the control of the mouse HoxB7 promoter. The Cre recombinase expression is detected in collecting ducts of ureteric bud descendants.
Contact: info@pkd-rrc.org

Mks5 Null

Allele Description: Null allele of Mks5 (rpgrip1l; nphp8)
Phenotype Description: Heterozygous mutant zebrafish are viable. Some of the homozygous mutant zebrafish survive and some have slight spine curvature defects. These viable mutants have reduced fertility. Maintained as a heterozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Nphp1 Null

Allele Description: Null allele #1 of Nphp1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org

Ift172 Null

Strain: Ift172
ZFIN ID:ZDB-GENE-040827-1
Allele designation:Δ1 bp +12 bp; frameshift in exon 9
Allele: Null allele of Ift172
Background: AB
Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org