Nphp1 Null
Allele Description: Null allele #1 of Nphp1
Phenotype Description: Homozygous mutant zebrafish are viable. Maintained as a homozygous stock.
Allele: Null
Background: AB
Contact: info@pkd-rrc.org
Ift172 Null
/in In Vivo PKD Models and Reagents, Zebrafish IFT Alleles, Zebrafish PKD Cilipathy ModelsStrain: Ift172
ZFIN ID:ZDB-GENE-040827-1
Allele designation:Δ1 bp +12 bp; frameshift in exon 9
Allele: Null allele of Ift172
Background: AB
Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org
Nek8 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsAllele Description: Null allele of Nek8
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutant zebrafish are embryonic lethal. Maintained as a heterozygous stock
Background: AB
Contact: info@pkd-rrc.org
Ift27 LacZ Null
/in Hepato-Renal Fibrocystic IFT Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a lacZ cassette and a Neomycin gene inserted in the second intron of the Ift27 gene. This allele expresses LacZ under the endogenous Ift27 promoter resulting in a null allele. Heterozygous mice are phenotypically normal. Homozygous mice are embryonic lethal.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Nek8 or jck
/in Cilia Transition Zone Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model harbors a double nucleotide substitution in the Nek8 gene. Heterozygous mice are phenotypically normal. Homozygous mice develop cysts in the kidney at approximately 21 days after birth and die at 4 months of age.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Ift88 flox
/in Hepato-Renal Fibrocystic IFT Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This conditional floxed mouse model harbors LoxP sites flanking exons 4 through 6 of the Ift88 gene. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of Ift88 in the Cre targeted tissue. This mouse model may be useful to study human genetic ciliopathies.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
mTmG Reporter: Expresses dTomato ubiquitously before Cre expression and GFP after recombination
/in In Vivo PKD Models and Reagents, Mouse Biosensor and Reporter Lines, Mouse Cre Lines, Reporters, and BiosensorsDescription: This mouse reporter line harbors a double-fluorescent Cre reporter that widely expresses the membrane-targeted tandem dimer Tomato (mT) in all cells/tissue. After Cre recombination the red mT cassette is deleted and the membrane-targeted green fluorescent protein (mG) is expressed in the Cre targeted cells/tissue. These mice are phenotypically normal.
Contact: info@pkd-rrc.org
IRG Reporter: Expresses dsRed ubiquitously before Cre expression and GFP after recombination
/in In Vivo PKD Models and Reagents, Mouse Biosensor and Reporter Lines, Mouse Cre Lines, Reporters, and BiosensorsDescription: This mouse reporter line harbors a double-fluorescent Cre reporter allele with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following Cre-mediated recombination. The allele is functionally similar to mTmG, but does not reside in the Rosa26 locus. These mice are phenotypically normal.
Contact: info@pkd-rrc.org
Mks1 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsStrain: Mks1
ZFIN ID:ZDB-GENE-030131-3813
Allele designation: Insertion of 2 bp, frameshift in exon 2
Allele: Null allele of Mks1
Background: AB
Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org
Mks2 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsStrain: Mks2 (tmem216)
ZFIN ID:ZDB-GENE-100818-1
Allele designation:Δ8 bp; frameshift in exon 4
Allele Description:Null allele of Mks2 (tmem216)
Phenotype Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Background: AB
Contact: info@pkd-rrc.org
Mks3 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsAllele Description: Null allele of Mks3 (tmem67)
Phenotype Description: Heterozygous mutant zebrafish are viable. Majority of homozygous mutants are embryonic lethal, however some live and have spine curvature defects. These viable mutants are infertile. Maintained as a heterozygous stock
Allele: Null
Background: AB
Contact: info@pkd-rrc.org
Mksr1 (B9d1) Flox
/in Cilia Transition Zone Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This conditional Mksr1 (B9d1) floxed mouse model harbors LoxP sites flanking exon 3 of the Mksr1 gene. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of Mksr1 in the Cre targeted tissue.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Mks6 LacZ Null
/in BBSome Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a lacZ cassette and a Neomycin gene inserted in intron 5 of the Mks6 (Cc2d2a) gene that disrupts expression of the Mks6 protein.
Contact:info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Mksr2 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsStrain: B9d2 (mksr2)
ZFIN ID:ZDB-GENE-040718-90
Allele designation: Insertion of 16 bp, frameshift in exon 2
Allele: Null allele of Mksr2 (B9d2)
Background: AB
Description: Heterozygous mutant zebrafish are viable. Majority of homozygous mutants are embryonic lethal, however some live and have spine curvature defects. These viable mutants are infertile. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org
Cep164 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsStrain:Cep164
ZFIN ID:ZDB-GENE-120821-1
Allele designation:Δ11 bp; frameshift in exon 1
Allele: Null allele of Cep164
Background: AB
Description: Cep164 homozygous mutant zebrafish are viable, fertile adults.
Contact: info@pkd-rrc.org
PKD2fl; Pax8; Tet-O-Cre + & Cre – (Late Induction)
/in In Vivo PKD Models and Reagents, PKD2 Mouse BiospecimensInduction/Description: P27/Doxycycline
Endpoint: 8weeks, 14weeks, 18weeks and 22weeks (Other timepoints can be generated upon request)
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
Cep290 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsStrain: Cep290
ZFIN ID:ZDB-GENE-041111-243
Allele designation:Δ23 bp; frameshift in exon 5
Allele: Null allele of Cep290
Background: AB
Description: Heterozygous mutant zebrafish are viable. Majority of homozygous mutants are embryonic lethal, however some live and have spine curvature defects. These viable mutants have reduced fertility. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org
CiliaGFP: Expresses GFP-tagged SSTR3 in all cilia
/in In Vivo PKD Models and Reagents, Mouse Biosensor and Reporter Lines, Mouse Cre Lines, Reporters, and BiosensorsDescription: This knock-in mouse model carries a floxed STOP cassette upstream of a cilia-localized somatostatin receptor 3 (SSTR3) protein fused to GFP that results in a ubiquitous green fluorescent cilia after Cre recombination. These mice are phenotypically normal.
Contact: info@pkd-rrc.org
Ift38 LacZ (Cluap1) Null
/in Hepato-Renal Fibrocystic IFT Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a lacZ: Neomycin fusion cassette inserted in intron 2 and LoxP sites flanking exon 3 of the Ift38 gene (aka Cluap1) resulting in a null allele. Heterozygous mice are phenotypically normal. Homozygous mice are embryonic lethal.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
cpk Heterozygous
/in Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This knock-out mouse model has a spontaneous tandem deletion of 12 bp and 19 bp in the cpk gene that results in a frameshift mutation within exon 1, truncating cystin, the protein encoded by the cpk gene. Heterozygous mice are phenotypically normal. Homozygous mice develop rapidly progressive renal cystic disease that closely resembles human autosomal recessive PKD (ARPKD) and die by 3-4 weeks of age. C57Bl6 lack the intrahepatic biliary duct dilatation observed in different backgrounds.
Contact: pydnam@uab.edu
Note: Additional combinations of alleles are available upon request
Gas8 Null
/in In Vivo PKD Models and Reagents, Motile Cilia Alleles, Zebrafish PKD Cilipathy ModelsStrain: Gas8
ZFIN ID:ZDB-GENE-030131-1980
Allele designation:Δ4 bp; frameshift in exon 9
Allele: Null allele of Gas8
Background: AB
Description: Heterozygous mutant zebrafish are viable. Homozygous mutants are embryonic lethal. Maintained as a heterozygous stock.
Contact: info@pkd-rrc.org
PKD2fl; PKHD1 Cre + and Cre –
/in In Vivo PKD Models and Reagents, PKD2 Mouse BiospecimensInduction/Description: Constitutively Active
Endpoint: P15, P30, P60
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
GCaMP6F Ca2+ Biosensor: Expresses a cytosolic Ca2+ reporter
/in In Vivo PKD Models and Reagents, Mouse Biosensor and Reporter Lines, Mouse Cre Lines, Reporters, and BiosensorsDescription:This mouse model harbors a Rosa-CAG-LSL-GCaMP6f conditional allele, designed with a floxed-STOP cassette upstream of the GCaMP6 fast variant calcium sensor. After Cre recombination a bright EGFP cytosolic fluorescence is observed following calcium binding to the reporter. These mice are phenotypically normal.
Contact: info@pkd-rrc.org
Bbs5 Null
/in Cilia Transition Zone / Cilopathy Alleles, In Vivo PKD Models and Reagents, Zebrafish PKD Cilipathy ModelsAllele Description: Null allele of Bbs5
Phenotype Description: Heterozygous mutant zebrafish are viable. Majority of homozygous mutant zebrafish are lethal, however some live and have spine curvature defects. These viable mutants do not produce progeny by natural mating. Maintained as a heterozygous stock
Background: AB
Contact: info@pkd-rrc.org
Bbs5 flox
/in BBSome Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This conditional Bbs5 floxed mouse model harbors LoxP sites flanking exons 3 through 4. Homozygous mice are phenotypically normal. When bred with a Cre recombinase mouse line, offspring lack expression of Bbs5 in the Cre targeted tissue.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Bbs5 LacZ
/in BBSome Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a lacZ cassette and a Neomycin gene inserted in intron 2 of the Bbs5 gene that disrupts expression of the Bbs5 protein. Heterozygous mice are phenotypically normal. Homozygous mice develop retinal defects and obesity.
Contact:info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
PKD1fl; KspCre + and Cre-
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: Constitutive
Endpoint: P3, P7, P14
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKD1fl; PKHD1 Cre + and Cre –
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: Constitutive
Endpoint: P15, P30, P60
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKD1V
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: Hypomorphic Allele
Endpoint: P3, P7, P14
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKD2 KO and control
/in In Vivo PKD Models and Reagents, PKD2 Mouse BiospecimensInduction/Description: Embryonically Lethal
Endpoint: E11.5, E12.5, E13.5, E14.5
Biomaterial available: Frozen Embryo
Contact: info@pkd-rrc.org
PKD2fl; KspCre + & Cre-
/in In Vivo PKD Models and Reagents, PKD2 Mouse BiospecimensInduction/Description: Constitutively Active
Endpoint: P3, P7, P14
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKD2fl; Pax8; Tet-O-Cre + & Cre – (Early Induction)
/in In Vivo PKD Models and Reagents, PKD2 Mouse BiospecimensInduction/Description: P10/Doxycycline
Endpoint: P15, P20, P25 (Other timepoints can be generated upon request)
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
Mksr1 LacZ (B9d1) Null
/in Cilia Transition Zone Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a lacZ cassette and a Neomycin gene inserted in intron 2 of the Mksr1 (B9d1) gene that disrupts expression of the Mksr1 protein.
Contact:info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
Ift140 LacZ Null
/in Hepato-Renal Fibrocystic IFT Alleles, Hepato-Renal Fibrocystic Rodent Models, In Vivo PKD Models and ReagentsDescription: This mouse model carries a LacZ knock-in (null) allele of Ift140. Ift140 is a component of the IFT complex A, which results in shortened, bulging cilia when mutated.
Contact: info@pkd-rrc.org
Note: Additional combinations of alleles are available upon request
PKD1 Ko & Control
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: Embryonically Lethal
Endpoint: E11.5, E12.5, E13.5, E14.5
Biomaterial available: Frozen Embryo
Contact: info@pkd-rrc.org
PKD1fl; Pax8; Tet-O-Cre + and Cre – (Early Induction)
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: P10/Doxycycline
Endpoint: P15, P20, P25 (Other timepoints can be generated upon request)
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKD1fl; Pax8; Tet-O-Cre + and Cre – (Late Induction)
/in In Vivo PKD Models and Reagents, PKD1 Mouse BiospecimensInduction/Description: P27/Doxycycline
Endpoint: 8weeks, 14weeks, 18weeks and 22weeks (Other timepoints can be generated upon request)
Biomaterial available: Frozen kidney, Frozen liver, Plasma, Paraffin Block
Contact: info@pkd-rrc.org
PKHD1 (pck) Rat
/in In Vivo PKD Models and Reagents, PKHD1 Rat Biospecimens, PKHD1 Single Allele Strains Rat ModelsDescription: These rats contain a mutation in the Pkhd1 gene. Homozygous mutant rats develop pathologies in both the liver and kidney while heterozygous animals are normal. This rat serves as a model of human autosomal-recessive polycystic kidney disease (ARPKD). The MTA for the PCK Rat must be obtained through Charles River. This line is cryopreserved at Charles River; however, live animals can be provided by UAB once the MTA is signed.
Contact: info@pkd-rrc.org
