Source: UMSOM

Description: A mouse inner medullary collecting duct cell line that contains a single stably integrated FRT site (for homologous intermolecular recombination) at a transcriptionally active genomic locus and the tetracycline repressor (which will enable you to regulate the activity of the gene of interest under the control of a tetracycline-regulated CMV/TetO2 promoter). The system allows the generation of stable cell lines exhibiting tetracycline-inducible expression of a gene of interest from a specific genomic location.

Available cell lines:

• mIMCD3-Flp-IN T-Rex cells with Pkd1 KO
• mIMCD3-Flp-IN T-Rex cells with Flag-tagged PKD1 overexpression
• Pkd1 wild-type MEFs
• Pkd1 KO MEFs

Important: When you submit your request, please enter the name of the cell line you are requesting in the Special Instructions.

Contact: info@pkd-rrc.org