CRISPR Vectors for Deletion of Human PKD2 Genes

The vectors express human U6 promoter driven crRNAs (gRNA sequence for each included in vector map) and FLAG-Cas9GFP under the control of a chicken beta actin promoter and CMV enhancer, allowing FACS sorting of transfected cells.

IMPORTANT: Please note the vector you are requesting from the diagrams below. When you submit your request, please enter the vector PX458-PKD2 Exon3_gRNA1, PX458-PKD2 Exon3_gRNA2 or PX458-PKD2 Exon2_gRNA3 in the Special Instructions.

Contact: info@pkd-rrc.org

CRISPR Vectors for Deletion of Mouse Genes

The vectors express human U6 promoter driven crRNAs (gRNA sequence for each included in vector map) and FLAG-Cas9GFP under the control of a chicken beta actin promoter and CMV enhancer, allowing FACS sorting of transfected cells. Download SnapGene Viewer to view the vector maps and sequences.

IMPORTANT: Please note the vector you are requesting from the diagram below. When you submit your request, please enter the vector Ift88 (pCJ1021), Pkd2 (pCJ1022), Ift88 + Pkd2 (pCJ1023) in the Special Instructions.

Contact: info@pkd-rrc.org

Special Orders

Special orders are non-standard resources such as tissues or organs that can have specific timepoints the requestor is in need of. Click on the “Add to Request” tab and once on the view cart page, additional information can be added to the special instructions box.

Contact: info@pkd-rrc.org

PC2 CT

Description: Rabbit polyclonal antibody directed to human PC2’s cytosolic C-terminal tail. 100uL per aliquot

Contact: info@pkd-rrc.org

PC1-NTF E3/4

Description: This is a mouse monoclonal antibody directed to human PC1’s C-lectin domain. 100ug per aliquot

Contact: info@pkd-rrc.org

PC1-NTF LRR

Description: Rabbit polyclonal antibody directed to human PC1’s LRR domain. 100uL per aliquot

Contact: info@pkd-rrc.org

PC1-NTF E1/2

Description: This is a mouse monoclonal antibody directed to human PC1’s LRR domain. 100ug per aliquot

Contact: info@pkd-rrc.org

PC1-CTF E8

Description: This is a rat monoclonal antibody directed to mouse PC1’s TOP domain. 100ug per aliquot

Contact: info@pkd-rrc.org

PC1-CTF rCC

Description: This is a rabbit polyclonal antibody directed to mouse PC1’s cytosolic C-terminal tail. 100uL per aliquot

Contact: info@pkd-rrc.org

PC1-CTF cCC

Description: This is a chicken polyclonal antibody directed at mouse PC1’s cytosolic C-terminal tail. 100uL per aliquot. 

Contact: info@pkd-rrc.org

CRISPR Vectors for Mouse PKD Gene knock-outs

IMPORTANT: Please note the vector you are requesting in the diagram below. When you submit your request, please enter the vector (PKD1, PKD2, or PKHD1) in the Special Instructions.

Contact: info@pkd-rrc.org

 

PKHD1 wild type and mutant expression constructs

IMPORTANT: Please note the construct number you are requesting in the diagram below. When you submit your request, please enter the construct number in the Special Instructions.

Contact: info@pkd-rrc.org

 

PKD2 wild type and mutant expression constructs

IMPORTANT: Please note the construct number you are requesting in the diagram below. When you submit your request, please enter the construct number in the Special Instructions.

Contact: info@pkd-rrc.org

 

PKD1 wild type and mutant expression constructs

IMPORTANT: Please note the construct number you are requesting in the diagram below. When you submit your request, please enter the construct number in the Special Instructions.

Contact: info@pkd-rrc.org

 

Cloning Kit

Contact: info@pkd-rrc.org

Antibody Diagram 2 (PD1 abs)