Archive for: Mouse Kidney Cell Lines

Source Institution: UAB
Allele Designation: Wild type, SV40
Source: These cell were derived from renal cortex of a 14 year old boy and then immortalized with pLenti-SV40-T-tsA58. A single cell was subcloned to generate a clonal population.
Cell Type: Renal epithelial cells, cortex. These cells express markers of collecting duct principal cells.
Purpose: Valuable normal human renal epithelial model for studying PKD or other renal diseases. These cells have a stable Karyotype.

Contact: info@pkd-rrc.org

Source Institution: UMSOM
Allele Designation: PKD1, Pax8rtTA-Cre, SV40
Source: Conditional PKD1 mouse expressing Pax8rtTA-Cre, crossed to the Immortomouse
Cell Type: Renal epithelial cell, Proximal Tubule
Purpose: Inducible knockout of PKD1 with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Source Institution: UMSOM
Allele Designation: PKD1
Source: Murine IMCD3 (mIMCD3) cell line containing genetically encoded Flp-In T-Rex system and a CRISPR knock out of PKD1
Cell Type: Renal epithelial cell, IMCD
Purpose: This cell line can be used to generate stable and tetracycline inducible gene expression in a PKD1 null background. It can be used to express mutant forms of PKD1.

Source Institution: UMSOM
Allele Designation: PKD2, Pax8rtTA-Cre, SV40
Source: Fibroblasts are derived from E 12.5 PKD2 null embryos. Controls fibroblasts are from wild type littermates, genetic background C57BL/6J
Cell Type: Mouse embryonic fibroblast (MEF) cells, Passage P0
Purpose: PKD2 null mouse embryonic fibroblasts paired with wild type control

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Ift88 mouse, expressing CAGG-CreER (tamoxifen inducible Cre), crossed to the Immortomouse
Cell Type: Renal epithelial cell, collecting duct
Purpose: Inducible knockout of Ift88, an essential ciliary protein, with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.