Immortalized human renal epithelial cells (Clone UABhk1-13)

Source Institution: UAB
Allele Designation: Wild type, SV40
Source: These cell were derived from renal cortex of a 14 year old boy and then immortalized with pLenti-SV40-T-tsA58. A single cell was subcloned to generate a clonal population.
Cell Type: Renal epithelial cells, cortex. These cells express markers of collecting duct principal cells.
Purpose: Valuable normal human renal epithelial model for studying PKD or other renal diseases. These cells have a stable Karyotype.

Contact: info@pkd-rrc.org

Immortalized mouse PKD1fl/fl, Pax8rtTA-Cre cells (Clone #199)

Source Institution: UMSOM
Allele Designation: PKD1, Pax8rtTA-Cre, SV40
Source: Conditional PKD1 mouse expressing Pax8rtTA-Cre, crossed to the Immortomouse
Cell Type: Renal epithelial cell, Proximal Tubule
Purpose: Inducible knockout of PKD1 with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse PKD2fl/fl, Pax8rtTA-Cre cells (Clone #125)

Source Institution: UMSOM
Allele Designation: PKD2, Pax8rtTA-Cre, SV40
Source: Conditional PKD2 mouse expressing Pax8rtTA-Cre, crossed to the Immortomouse
Cell Type: Renal epithelial cell, IMCD
Purpose: Inducible knockout of PKD2 with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse PKD1fl/fl, Pax8rtTA-Cre cells (Clone #187)

Source Institution: UMSOM
Allele Designation: PKD1, Pax8rtTA-Cre, SV40
Source: Conditional PKD1 mouse expressing Pax8rtTA-Cre, crossed to the Immortomouse
Cell Type: Renal epithelial cell, collecting duct
Purpose: Inducible knockout of PKD1 with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse Ift88fl/fl, Cre negative, renal epithelial cells (Clone BY1107)

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Ift88 mouse lacking Cre expression, crossed to the Immortomouse
Cell Type: Renal epithelial cell
Purpose: Murine renal epithelial cell line that serves as a control (tamoxifen-treatment) for Clone BY2574
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse Ift88fl/fl, Cre negative, MEF cells (Clone BY6458)

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Ift88 mouse, Cre negative, crossed to the Immortomouse
Cell Type: Mouse embryonic fibroblast (MEF) cells
Purpose: MEF cell line that serves as a control (tamoxifen-treatment) for Clone BY2847
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse Ift88fl/fl, tamoxifen inducible Cre, MEF cells (Clone BY2847)

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Ift88 mouse expressing CAGG-CreER (tamoxifen inducible Cre), crossed to the Immortomouse
Cell Type: Mouse embryonic fibroblast (MEF) cells
Purpose: MEF cell line that serves as a control (tamoxifen-treatment) for Clone BY2847
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Primary mouse PKD2fl/fl, Pax8rtTA-Cre mTmG cells

Source Institution: UMSOM
Allele Designation: PKD2, Pax8rtTA-Cre, mTmG, SV40
Source: Conditional PKD2 mouse expressing Pax8rtTA-Cre and mT (tomato) mG (green fluorescent protein) dual-fluorescent Cre reporter
Cell Type: Renal epithelial cell, mixed population
Purpose: Primary murine cells with inducible knockout of PKD2 expressing mTmG to visualize Cre activity

Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Primary mouse PKD1fl/fl, Pax8rtTA-Cre, mTmG cells

Source Institution: UMSOM
Allele Designation: PKD1, Pax8rtTA-Cre, mTmG, SV40
Source: Conditional PKD1 mouse expressing Pax8rtTA-Cre and mT (tomato) mG (green fluorescent protein) dual-fluorescent Cre reporter
Cell Type: Renal epithelial cell, mixed population
Purpose: Primary murine cells with inducible knockout of PKD1 expressing mTmG to visualize Cre activity
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Contact: info@pkd-rrc.org

Immortalized mouse PKD1V/V cells (Clone #21)

Source Institution: UMSOM
Allele Designation: PKD1V/V
Source: PKD1V/V mouse crossed to the Immortomouse
Cell Type: Renal epithelial cell, collecting duct
Purpose: Knock-in mutation at PKD1 GPS cleavage site. This mutation induces cystic disease
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Immortalized mouse wildtype cells (Clone #12)

Source Institution: UMSOM
Allele Designation: Wild type
Source: Immortomouse
Cell Type: Renal epithelial cell, collecting duct, distal tubule

mIMCD3 Flp-In T-Rex

Source Institution: UMSOM
Allele Designation: Wild type
Source: Murine IMCD3 (mIMCD3) cell line containing genetically encoded Flp-In T-Rex system.
Cell Type: Renal epithelial cell, IMCD
Purpose: This cell line can be used to generate stable and tetracycline inducible gene expression. This line can also be used as a control for mIMCD3 Flp-In T-Rex PKD1 KO

mIMCD3 Flp-In T-Rex PKD1 KO

Source Institution: UMSOM
Allele Designation: PKD1
Source: Murine IMCD3 (mIMCD3) cell line containing genetically encoded Flp-In T-Rex system and a CRISPR knock out of PKD1
Cell Type: Renal epithelial cell, IMCD
Purpose: This cell line can be used to generate stable and tetracycline inducible gene expression in a PKD1 null background. It can be used to express mutant forms of PKD1.

PKD1 Null Mouse Embryonic Fibroblasts and control

Source Institution: UMSOM
Allele Designation: PKD1
Source: Fibroblasts are derived from E 12.5 PKD1 null embryos. Controls fibroblasts are from wild type littermates,genetic background C57BL/6J
Cell Type: Mouse embryonic fibroblast (MEF) cells, Passage P0
Purpose: PKD1 null mouse embryonic fibroblasts paired with wild type control

PKD2 Null Mouse Embryonic Fibroblasts and control

Source Institution: UMSOM
Allele Designation: PKD2, Pax8rtTA-Cre, SV40
Source: Fibroblasts are derived from E 12.5 PKD2 null embryos. Controls fibroblasts are from wild type littermates, genetic background C57BL/6J
Cell Type: Mouse embryonic fibroblast (MEF) cells, Passage P0
Purpose: PKD2 null mouse embryonic fibroblasts paired with wild type control

Immortalized mouse Kif3afl/fl, tamoxifen inducible Cre, CD cells (Clone BY176-6C)

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Kif3a mouse expressing CAGG-CreER (tamoxifen inducible Cre), crossed to the Immortomouse
Cell Type: Renal epithelial cell, collecting duct
Purpose: Inducible knockout of Kif3a, an essential ciliary protein, with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Immortalized mouse Ift88fl/fl, tamoxifen inducible Cre, CD cells (Clone BY2574)

Source Institution: UAB
Allele Designation: Ift88, SV40
Source: Conditional Ift88 mouse, expressing CAGG-CreER (tamoxifen inducible Cre), crossed to the Immortomouse
Cell Type: Renal epithelial cell, collecting duct
Purpose: Inducible knockout of Ift88, an essential ciliary protein, with isogenic control cells
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.

Immortalized mouse Ift88fl/fl, Cre negative expression, renal epithelial cells (Clone BY1106)

Source Institution: UAB
Allele designation: IFT88, SV40
Source: Conditional Ift88 mouse, Cre negative, crossed to the Immortomouse
Cell Type: Renal epithelial cell
Purpose: Murine renal epithelial cell line that serves as a control (tamoxifen-treatment) for Clone BY2574
Note: Mouse embryonic fibroblasts are available from any mouse model upon request.